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Fingernail Testing

What do professionals who use fingernail testing for drugs and alcohol know that you don't?

Some of the most frequently asked questions are related to the types of tests and what they are used for.  

Fingernail, made of keratin like hair, is emerging as a popular specimen type for drug and alcohol testing.  It is simple to collect, easy to ship and store, and is at the cutting edge of drug and alcohol testing.  

Drug and alcohol biomarkers are trapped in the fingernail by three main routes:

1) Blood flow in the germinal matrix deposits drug and alcohol biomarkers into the nail when it is formed.

2) Blood flow in the nail bed deposits drug and alcohol biomarkers in the nail as it thickens.

3) Sweat and oil of the skin surrounding the nail deposits drug and alcohol biomarkers into the nail.

These three very different routes of incorporation are superimposed on top of each other rendering a very complex drug history picture.

1:1 Nail and hair samples capture the same drugs

Non-intrusive, donor collected sample. Fingernails are collected by the donor in front of a trained staff member.

Minimal impact on the donors appearance.

All samples are screened on state -of-the-art instruments, and positives are confirmed on GC/LC MS before reported out.

Window of Detection & Collection

Nails provide up to 6 months of drug use history and up to 3 months of alcohol history. Biomarkers are detectable in nail as early as one week after drug or alcohol use.  The window of detection for drug or alcohol ingestion depends on the substance used, the amount used, and personal metabolism.  Just like hair and urine, a negative result is not proof of abstinence, just the lack of evidence. 

A 2-3 mm clipping, about the thickness of a quarter, from the end of each fingernail provides the optimal sample. Not enough fingernail?  Wait 1-2 weeks.  What is positive today will still be positive in 2 weeks.  Never mix fingernails and toenails in the same sample. 

How nails grow is important for biomarker testing

The nail originates in the germinal matrix and grows outward toward the fingertip.  The hardened material forming the nail plate grows across the nail bed, which is rich in capillary blood flow.  As the nail grows in length, material is added from underneath such that the nail lengthens and thickens, as it grows outward towards the fingertip.  The portion of the nail extending past the fingertip is called the free edge, the piece that you clip when clipping your nails.  The entire process takes up to 6 months depending on the health of the individual.  

Drug and alcohol biomarkers are trapped in the keratin fibers of the fingernail

Hair is also made of keratin fibers, but biomarkers may be washed out of hair by common cosmetic treatments such as bleaches, dyes, permanents and straighteners, reducing or eliminating the presence of the detectable substances.  This problem does not exist when testing nails. 

Nail keratin is 4x thicker than hair

Nail often captures more of a biomarker than hair.  

As nail grows in thickness and length, biomarkers build up in the nail at the germinal matrix and along the nail bed, collecting the full history of drug use.  

Our services are not intended to diagnose, treat, cure or prevent any disease.  For forensic use only.

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